Year 2004 2006 2008 2010 2012 2014 2015 2016 2018
Wave 1 2 3 4 5 6 CheckPoint 7 8
Age 3-19 months 2-3 years 4-5 years 6-7 years 8-9 years 10-11 years 11-12 years 12-13 years 14-15 years
Biomarker analysis
(urine, albumin and creatinine,
albumin-to-creatinine ratio) 1 2
X
Biomarker analysis
(plasma, high sensitivity C-Reactive Protein – hsCRP) 3
X
Metabolic profile
(serum, Nightingale 2017 panel) 4
X
Metabolic profile
(plasma, hydrosoluble vitamins,
lipid-soluble vitamins, one-carbon metabolites)
X
Genome-wide SNP genotyping 5 X
Telomere length 6 X

Notes

1 Albumin and creatinine were quantified in urine to assess for albuminuria. A Cobas Integra® 400 plus analyzer, determining albumin using an immunoturbimetric assay (ALBT2 kit, Test ID 0-171, Roche Diagnostics, Germany), and creatinine using the enzymatic colorimetric method (Creatinine plus version2 CREP2U kit, Test ID 0-512, Roche Diagnostics, Germany). Laboratory work undertaken at the Baker Heart and Diabetes Institute (Melbourne, Australia) in 2016
2 Female participants were asked their menstruation status on the day of testing
3 hsCRP was measured using ultra-performance liquid chromatography– tandem mass spectrometry (UPLC–MS/MS)
4 The Nightingale® Nuclear Magnetic Resonance (NMR) metabolomics platform was used to quantify 288 metabolic biomarkers from 0.35mL of serum using the 2017 version quantification of Nightingale’s algorithm (www.nightingalehealth.com). Key variables include total cholesterol, LDL cholesterol, HDL cholesterol, total triglycerides, glucose, glycoprotein acetyls, apolipoprotein A1 (apoA1), and apolipoprotein B (apoB)
5 The Illumina Infinium Global Screening Array v2.0 was used to perform genome-wide genotyping
6 Telomere length, related to cellular ageing, is expressed as a ratio of telomere repeat length to copy number of a single copy gene. Genomic DNA was extracted from a single 0.5ml whole blood aliquot or single clot aliquot (where whole blood was not available) per participant, using a QIAcube workstation and QIAamp DNA Blood Kit (Qiagen) according to the kit protocol. Relative telomere length was assessed using quantitative PCR (Roche LightCycler 480).

Legend

A = Administration report abstraction
C = Index child report (reporting on others)
L = Linkage (to other databases)
M = Medical records data abstraction
O = Observation/direct assessment
N = Nurse report
P = Parent/primary caregiver report
Pe = Peer report
S = Self report
T = Teacher report
X = Undefined
X BF = Pertaining to biological father
X BM = Pertaining to biological mother
C = Pertaining to index child
X F = Pertaining to father
X Fam = Pertaining to family
X G = Pertaining to grandparent(s)
X M = Pertaining to mother
X P = Pertaining to parent(s)
X Pa = Pertaining to partner
X Pe = Pertaining to peers
X Pr = Pertaining to primary caregiver
X Si = Pertaining to sibling(s)